Luke Shaw, Adam Lallana and Joe Gomez have all returned to the England squad, while Gareth Southgate has included uncapped goalkeeper Alex McCarthy.
United change | Shaw replaces Young
The Southampton stopper gets the nod along with Jack Butland and number one goalkeeper Jordan Pickford, while Shaw takes the place of Manchester United team-mate Ashley Young.
Gary Cahill and Jamie Vardy are not included after both players indicated they would be taking a step back from international duty, although they have not retired.
England face Spain in their Nations League opener on September 8 and take on Switzerland in an international friendly three days later.
Southgate has kept faith with the majority of the 23-man squad that reached the semi-finals of the World Cup, with 18 players retaining their places.
There is also a spot for Burnley defender James Tarkowski, who was on the standby list for the tournament before pulling out to undergo hernia surgery.
Manchester United defender Phil Jones and Tarkowski's team-mate Nick Pope are among those who went to Russia 2018 but are not included due to injury.
England squad in full:
Jack Butland (Stoke City), Alex McCarthy (Southampton) Jordan Pickford (Everton); Trent Alexander-Arnold, Joe Gomez (both Liverpool), Harry Maguire (Leicester City), Danny Rose (Tottenham), Luke Shaw (Manchester United), John Stones (Everton) James Tarkowski (Burnley), Kieran Trippier (Tottenham), Kyle Walker (Manchester City); Dele Alli (Tottenham), Fabian Delph (Manchester City); Eric Dier (Tottenham), Jordan Henderson (Liverpool), Adam Lallana (Liverpool), Jesse Lingard (Manchester United), Ruben Loftus-Cheek (Chelsea); Harry Kane (Tottenham), Marcus Rashford (Manchester United), Raheem Sterling (Manchester City), Danny Welbeck (Arsenal).