Poland's Szymon Marciniak, 38, was also the referee for Real Madrid's UEFA Super Cup defeat to city rivals Atlético back in August.
Poland's Szymon Marciniak has been appointed as the referee for Ajax and Real Madrid's Champions League last-16 first leg in Amsterdam on Wednesday.
Marciniak was referee for Madrid's Super Cup loss to Atlético
The 38-year-old, a FIFA international official since 2011, took charge of Madrid's 4-2 UEFA Super Cup defeat to Atlético Madrid in August, and last oversaw a Champions League game involving Los Blancos in the 2016/17 season, when the holders played out a 1-1 group-stage draw with Tottenham Hotspur at the Bernabéu.
Also the referee for Spain's 3-2 UEFA Nations League loss to England in October, Marciniak was handed four games in this season's Champions League group stage: AEK Athens 1-1 Videoton, Red Star 0-0 Napoli, Inter Milan 1-1 Barcelona and Paris Saint-Germain 2-1 Liverpool.
At last summer's World Cup in Russia, the Pole refereed two of the first round's most notable matches, Argentina 1-1 Iceland and Germany 2-1 Sweden, and took charge of three games at Euro 2016, including Spain's 1-0 group-stage win over the Czech Republic.
He also officiated Ajax's 2016/17 Europa League semi-final second leg against Lyon, which saw the Dutchmen reach the final in Solna - where they lost 2-0 to Manchester United - despite going down 3-1 on the night.
Mateu Lahoz to ref Spurs' first leg with Dortmund
Meanwhile, Tottenham's last-16 first leg at home to Borussia Dortmund, which also takes place on Wednesday, will be overseen by Spain's Antonio Mateu Lahoz.